(095) Contribution of Testicular Microbiome to the Seminal Microbiota: Are There Repercussions After Vasectomy?

Abstract Introduction The human microbiome has been implicated in a variety of clinical conditions. semen harvests compendium microorganisms that potentially originate different anatomic structures, such as the testis, prostate, and seminal vesicles. However, there is paucity data delineating significance male reproductive health, specifically testicular its role over microbiota. Objective To analyze changes after impeding paracrine migration caused by undergoing vasectomy. Methods Seminal samples were collected from 40 normozoospermic patients (normal concentration motility according to WHO parameters) 36 post-vasectomy (samples >3 months vasectomy, timeframe which expected have stabilized procedure). Sixteen gave pre- sample. Richness (α-diversity) microbiome, calculated number operational taxonomic units, clusterization (β-diversity), obtained Bray Curtis distances, compared between SS, then only among paired SS. relative abundance species, genera, phylum was groups using analysis compositions microbiomes with bias correction (ANCOM-BC). Secondarily, association age, BMI, ethnicity, recent antibiotic use (<3 before obtaining SS) profiling analyzed. Statistical assessed at p<0.05. Results Operational units (OTUs) found be higher group (median=35 OTUs) than (median=29 (p=0.018). Similarly, men who had samples, pre-vasectomy (median=43 α-diversity OUTs) (p<0.001), suggesting community heterogenicity groups. β-diversity similar (p=0.584), also (p=0.080) composition remained, majority, Nonetheless, when abundances prevalence less 10% analyzed, we 12 species 8 genera differed samples. Age, not associated composition. Conclusions Our study highlights without contribution due would exhibit fewer OTUs change genera. This causes modification semen, implying contributes configuration Further investigations are needed determine impact these findings male’s health. Disclosure Yes, this sponsored industry/sponsor: MicroGenDX Clarification Industry funding - investigator initiated executed Any authors act consultant, employee or shareholder an industry for: Acerus, Boston Scientific, Endo Pharmaceuticals Coloplast.